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Dual metastable peak monitoring: Application to the analysis of oestradiol‐17β as the bis ( tert ‐butyldimethylsilyl) ether
Author(s) -
Thorne Gareth C.,
Gaskell Simon J.
Publication year - 1985
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200120105
Subject(s) - ether , dual (grammatical number) , metastability , chemistry , chromatography , organic chemistry , art , literature
A new approach to dual metastable peak monitoring has been developed, based on synchronous switching of the accelerating voltage and electric sector voltage of a double‐focusing mass spectrometer. The technique has been applied to the determination of oestradiol‐17β as the bis( tert ‐butyldimethylsilyl) ether, using the 2 H 3 analogue as internal standard. The detection limit was approximately 5 pg during monitoring of the [M] +. →[M‐C 4 H 9 ] + fragmentation. Analyses of plasma extracts indicated that greater selectivity of detection was achieved during metastable peak monitoring than during high resolution (8000) selected ion monitoring of the parent ion.