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CAD MIKES: A new method for a rapid and unequivocal structural identification of organic acids in biological fluids. A first application to a case of methylmalonic aciduria
Author(s) -
Rinaldo Piero,
Chiandetti Lino,
Zacchello Franco,
Daolio Sergio,
Traldi Pietro
Publication year - 1984
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200111209
Subject(s) - methylmalonic acid , chemistry , urine , chromatography , mass spectrometry , biochemistry , homocysteine
A novel application of Collisionally Activated Decomposition Mass analysed Ion Kinetic Energy (CAD MIKE) spectrometry to separation and positive structural identification of urinary methylmalonic acid (MMA) (the pathognomonic compound for the diagnosis of methylmalonic acidurias) is presented. CAD MIKES scans of EI ionic species at m / z 119 ([M + H] + ) and m / z 101 ([M – OH] + ) have been obtained from a pure standard of MMA and from crude urinary acid fractions. With reference to the procedures employed so far, the advantages of the proposed method lie in fast and simplified sample pretreatment and in a quick non‐controversial response to a clinical suspicion of serious, life‐threatening inherited metabolic diseases.