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Fast atom bombardment mass spectrometry and tandem mass spectrometry in antibiotics: Identification of nucleoside antitumor antibiotic toyocamycin in fermentation broth
Author(s) -
Tondeur Yves,
Shorter Mary,
Gustafson M. E.,
Pandey R. C.
Publication year - 1984
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200111206
Subject(s) - mass spectrometry , fast atom bombardment , chemistry , tandem mass spectrometry , chromatography , fermentation , nucleoside , biochemistry
The presence of the nucleoside antitumor antibiotic toyocamycin in the fermentation broth was determined by a combination of negative and positive ion fast atom bombardment (FAB) mass spectrometry, high resolution FAB mass spectrometry and mass‐analysed ion kinetic energy spectrometry (MIKES). A reasonable limit of detection for toyocamycin in the whole broth was obtained by combining the specificity of mass spectrometry/mass spectrometry (also called tandem mass spectrometry) to FAB. The role played by the fermentation matrix upon the production and the observation of characteristic ions by FAB. using xenon atoms was examined. High‐performance liquid chromatography (HPLC) and FAB mass spectrometry were used to monitor toyocamycin at all stages of strain development, fermentation and recovery.