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A comparison of thermospray and direct liquid introduction high‐performance liquid chromatography/mass spectrometry for the analysis of candidate antimalarials
Author(s) -
Voyksner Robert D.,
Bursey Joan T.,
Hines John W.,
Pellizzari Edo D.
Publication year - 1984
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200111205
Subject(s) - thermospray , chromatography , mass spectrometry , high performance liquid chromatography , chemistry , detection limit , analytical chemistry (journal) , liquid chromatography–mass spectrometry , tandem mass spectrometry , selected reaction monitoring
Abstract The analysis of antimalarials by high‐performance liquid chromatography (HPLC)/mass spectrometry demonstrates a new dimension in specificity along with increased sensitivity compared to conventional HPLC detection methods. Both direct liquid introduction and thermospray HPLC/mass spectrometry interfaces provided molecular weight information as well as characteristic fragment ions for antimalarials not normally amenable to direct probe or gas chromatographic/mass spectrometric techniques. The direct liquid introduction interface, which incorporated a 1/100 split, showed a detection limit of 30 ng using selected ion monitoring. The thermospray technique showed less than 1 ng detection limits using selected ion monitoring.