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A stable isotope method for the quantification of N ‐acetyl‐5‐aminosalicylic acid in plasma and urine
Author(s) -
Fischer C.,
Meese C. O.,
Klotz U.
Publication year - 1984
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200111009
Subject(s) - chemistry , urine , metabolite , aminosalicylic acid , acetylation , chromatography , in vivo , deuterium , biochemistry , pharmacology , biology , physics , microbiology and biotechnology , quantum mechanics , gene
Abstract The synthesis of a number of N ‐acyl‐5‐aminosalicylic acids and their derivatives is described. These compounds allow the sensitive and specific mass spectrometric determination of unlabelled or deuterium‐labelled N ‐acetyl‐5‐aminosalicylic acid in biological samples. An in vivo study shows that the labelled compound, administered to rats, is excreted isotopically unchanged to at least 97%, and that no significant deacety lation/acetylation mechanism exists for this metabolite N ‐acetyl‐5‐aminosalicylic acid of salicylazosulphapyridine.