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Application of fast atom bombardment mass spectrometry to biological samples: Analysis of urinary metabolites of acetaminophen
Author(s) -
Ackermann Bradley L.,
Watson J. Throck,
Newton John F.,
Hook Jerry B.,
Braselton W. Emmett
Publication year - 1984
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200111004
Subject(s) - chemistry , chromatography , mass spectrometry , fast atom bombardment , analyte , glucuronide , high performance liquid chromatography , mass spectrum , analytical chemistry (journal) , ionization , metabolite , ion , organic chemistry , biochemistry
Fast atom bombardment (FAB) is useful for the characterization of all major metabolites of the analgesic acetaminophen (APAP). It is particularly useful for providing mass spectra of the polar glucuronide and sulfate conjugates which eluded identification by field desorption and other more conventional methods of ionization. A protocol is described for the use of FAB in the identification of urinary APAP metabolites isolated by reversed phase high‐performance liquid chromatography (HPLC) following therapeutic dosages of the drug. A tentative set of recommendations for the off‐line use of HPLC and FAB is directed towards solving problems encountered when using these two analytical techniques in concert. In addition, a method for calculating the signal to background ratio (S/B) for analyte peaks in FAB spectra from selected relative ion intensities is proposed. Examples are presented that show the potential of S/B as an empirical parameter for judging the quality of FAB spectra.