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The analytical pyrolysis of carbohydrates. 2—differentiation of homopolyhexoses according to their linkage type, by pyrolysis–mass spectrometry and pyrolysis‐gas chromatography/mass spectrometry
Author(s) -
van der Kaaden Arie,
Boon Jaap J.,
Haverkamp Johan
Publication year - 1984
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200110910
Subject(s) - pyrolysis , pyrolysis–gas chromatography–mass spectrometry , mass spectrometry , chemistry , chromatography , analytical chemistry (journal) , gas chromatography , glycosidic bond , gas chromatography–mass spectrometry , organic chemistry , enzyme
Curiepoint pyrolysis (Py)‐mass spectrometry (14 eV electron impact) in various modes of operation and Curiepoint pyrolysis‐gas chromatograpy/mass spectrometry (Py‐GC/MS) afforded discrimination between homopolyhexoses of different glycosidic linkage type and configuration: (1 → 2)‐β‐, (1 → 3)‐β‐, (1 → 4)‐α‐, (1 → 4)‐β‐, (1 → 6)‐α, (1 → 6)‐β‐glucan and (1 → 2)‐α‐mannan. Pyrolysis‐mass spectrometry in multichannel averaging mode, combined with discriminant analysis data processing, revealed significant discrimination between all polyhexoses, except for the (1→6) linked polymers. By using preheated telescopic glass tube sample holders, additional specificity of the pyrolysis‐mass spectral fingerprint for the (1 → 3) linked carbohydrate was obtained. Pyrolysis‐mass spectrometry in time‐resolved mode yielded ion profiles, especially characteristic for the (1 → 3)‐β‐glucan. Py‐GC/MS was an additional discriminating dimension (retention time), providing structural information about the pyrolysate constituents, in particular concerning the anhydrohexoses.