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Development of a gas chromatographic selected ion monitoring assay for thyroxine (T4) in human serum
Author(s) -
Ramsden D. B.,
Farmer M. J.
Publication year - 1984
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200110811
Subject(s) - chromatography , chemistry , diiodotyrosine , mass spectrometry , gas chromatography , radioimmunoassay , selected ion monitoring , gas chromatography–mass spectrometry , biochemistry , hormone , triiodothyronine
The components of a gas chromatographic mass spectrometric‐selected ion monitoring (SIM) assay for thyroxine (T4) in human serum are described. The internal standard for the assay was synthesized from deuterium‐labelled 3,5‐diiodotyrosine and 3,5‐diiodo‐4‐hydroxyphenylpyruvic acid. A novel method was developed for isolating the products of the coupling reaction. The results obtained by gas chromatography mass spectrometry SIM were compared with those of radioimmunoassay. The gas chromatographic mass spectrometric SIM assay would form the basis of a reference assay for T4.