Premium
Structure determination of phospholipids by secondary ion mass spectrometric techniques: Differentiation of isomeric esters
Author(s) -
Ohashi Yoko
Publication year - 1984
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200110803
Subject(s) - chemistry , mass spectrum , ion , primary (astronomy) , secondary ion mass spectrometry , phospholipid , mass spectrometry , abundance (ecology) , matrix (chemical analysis) , fatty acid , analytical chemistry (journal) , chromatography , organic chemistry , biochemistry , physics , astronomy , membrane , fishery , biology
The secondary ion mass spectrometry, regardless of the charge status of the primary beam, is a good means not only of determining the type of phospholipid and the fatty acid components but also the isomeric positions of the acyl groups. Matrix‐assisted secondary ion (SI) mass spectra of phosphatidylcholines in the positive mode show two characteristic doublets 2 u apart originating from the deacyl and deacyloxy cleavages of the compounds. The relative abundance of these ions is diagnostic of the ester positions, that is, the fragments originating from the C 2 ‐ester loss are more abundant than the corresponding ones from the C 1 ‐ester loss.