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Chenodeoxycholic acid pool size determination from children using isotope dilution mass spectrometry
Author(s) -
Norman E. J.,
Heubi J. E.,
Gelfand M. J.,
Dan P.,
Matthews D. E.
Publication year - 1984
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200110603
Subject(s) - chenodeoxycholic acid , mass spectrometry , isotope dilution , chemistry , chromatography , isotope , bile acid , biochemistry , physics , quantum mechanics
An isotope dilution mass spectrometry method is described for determining chenodeoxycholic acid pool size in children. The stable isotopically labeled tracer. (11,12‐ 2 H 2 ) chenodeoxycholic acid, was administered orally to children, and the enrichment of bile was measured by selected ion monitoring gas chromatography mass spectrometry. The level of (11,12‐ 2 H 2 ) chenodeoxycholic acid enrichment found in the patient samples was in the range of 0.5 to 5%. Data are presented illustrating the duplication of this method in two independent laboratories using standard quadrupole mass spectrometers. This procedure provides the clinician with a non‐radioactive method for determining chenodeoxycholic acid pool size which is especially beneficial in studies involving children and pregnant women.