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Application of high resolution fast atom bombardment and constant B/E ratio linked scanning to the identification and analysis of acylcarnitines in metabolic disease
Author(s) -
Millington David S.,
Roe Charles R.,
Maltby David A.
Publication year - 1984
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200110508
Subject(s) - acetylcarnitine , chemistry , methylmalonic acidemia , fast atom bombardment , mass spectrometry , urine , carnitine , ion , isotope dilution , high resolution , resolution (logic) , chromatography , analytical chemistry (journal) , biochemistry , endocrinology , biology , remote sensing , organic chemistry , artificial intelligence , geology , computer science
Acylcarnitines, a biologically important group of metabolites which have thus far eluded characterization by mass spectrometry, exhibit very intense fast atom bombardment mass spectra dominated by parent cations. The combination of high resolution selected ion detection and daughter ion analysis using the linked scan at constant B/E ratio has enabled the unequivocal identification of the acylcarnitines in the urine of children with propionic acidemia, methylmalonic aciduria and Reye's Syndrome. Quantitative analysis of acetylcarnitine and propionylcarnitine in selected samples was accomplished by isotope dilution, utilizing ( 2 H 3 )acetyl‐ and ( 2 H 5 )propionylcarnitines as internal standards. These techniques will allow assessment of the therapeutic use of L‐carnitine in these disorders.