Premium
Investigations on basic aspects of fast atom bombardment mass spectrometry: Matrix effects, sample effects, sensitivity and quantification
Author(s) -
Lehmann W. D.,
Kessler M.,
König W. A.
Publication year - 1984
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200110505
Subject(s) - fast atom bombardment , chemistry , mass spectrometry , ion , matrix (chemical analysis) , analytical chemistry (journal) , atom (system on chip) , isotope , ionization , isotope dilution , chromatography , organic chemistry , physics , quantum mechanics , computer science , embedded system
Basic aspects f fast atom bombardment mass spectrometry such as matrix effects, discriminatory effects between positive and negative ion formation, sensitivity, and isotope dilution have been investigated using oligopeptides and amino acid derivatives as model compounds. The matrix selected was found to have a decisive influence on the sensitivity of fast atom bombvardment. Acidic and basic properties of the samples could be correlated with different ionization efficiencies in positive or negative ion formation. With respect to sensitivity, a roughly linear relation between signal intensity and sample amount was observed in the range of 1 ng to 10 μg of sample. Nanogram amounts of samples could be detected by fast atom bombardment mass spectrometry. Finally, the suitability of fast atom bombardment for quantitative investigations is proven by the investigation of isotopically labelled mixtures.