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A selected ion monitoring assay for chlorhexidine in medical waste water
Author(s) -
Matsushima Hajime,
Sakurai Nobuo
Publication year - 1984
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200110502
Subject(s) - trifluoroacetic anhydride , chlorhexidine , disinfectant , chromatography , chemistry , derivative (finance) , elution , wastewater , waste management , organic chemistry , medicine , dentistry , engineering , financial economics , economics
We have developed a method for determination of chlorhexidine, a disinfectant, in medical waste water by selected ion monitoring (SIM). Our method consists of the following procedures. (1) Chlorhexidine in the medical waste water was extracted and purified by an Extrelut® column with ethyl acetate. (2) The eluted chlorhexidine was converted to a triazine derivative with trifluoroacetic anhydride. (3) The triazine derivative was subjected to on‐column methylation on a gas chromatographic column. (4) Finally, the triazine N‐methyl derivative of chlorhexidine was identified and determined by SIM. Recoveries of the compound added to medical waste water were about 90% in amounts ranging from 1 to 10 μg. In the waste water from our medical waste water treatment plant, chlorhexidine concentrations were found to be 0.085–1.94 mg I −1 . The present method is superior to the pre‐existing ones in its quick separation, specificity and sensitivity.