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Conffirmatory assay for ivermectin in cattle tissue using chemical ionization mass spectrometry/mass spectrometry (MS/MS)
Author(s) -
Tway P. C.,
Dddowning G. V.,
Slayback J. R. B.,
Rahn G. S.,
Isersee R. K.
Publication year - 1984
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200110407
Subject(s) - mass spectrometry , ivermectin , chemistry , atmospheric pressure chemical ionization , mass spectrometry imaging , chromatography , ion mobility spectrometry–mass spectrometry , thermal ionization mass spectrometry , chemical ionization , ionization , selected reaction monitoring , tandem mass spectrometry , biology , ion , organic chemistry , zoology
A method based on direct exposure, positive ion, chemical ionization mass spectrometry/mass spectrometry (ms/ms) was developed for the confirmatory assay of the antiparasitic drug, ivermectin, in animal tissue. Following extraction, column and preparative liquid chromatography, mass spectrometric/mass spectrometric analysis of the drug in liver samples provided reliable detection limits to 8–10 parts‐par‐billion at a signal: noise of greater than 10:1. Blank tissue consistently displayed no chemical/matrix interference. Besides the development of a confirmatory assay, the study also demonstrates the analytical capabaility and the role of MS/MS vis‐a‐vis other applied mass spectrometric techniques.