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Analyses of lysophosphatidic acids by gas chromatography mass spectrometry without hydrolytic pretreatment
Author(s) -
Tokumura Akira,
Harada Kengo,
Yoshioka Yasuko,
Tsukatani Hiroaki,
Handa Yasomi
Publication year - 1984
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200110406
Subject(s) - chemistry , trimethylsilyl , silylation , mass spectrometry , lysophosphatidic acid , chromatography , gas chromatography , hydrolysis , gas chromatography–mass spectrometry , electron ionization , organic chemistry , biochemistry , ion , ionization , receptor , catalysis
Abstract Lysophosphatidic acids having diferent fatty acyl moieties were directly analysed by gas chromatography/mass spectrometry after silylation. Trimethylsilyl derivatives of various LPAs escaped thermal degradation, and electron impact and chemical ionization of these derivatives yielded many ions which were useful for the structural determination. It was found by this new method that five species of lysophosphatidic acids were formed in rat plasma during incubation at 37°C.