Premium
Analysis of moth pheromone acetates by selected ion monitoring using electron impact and chemical ionization mass spectrometry
Author(s) -
Löfstedt Christer,
Odham Göran
Publication year - 1984
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200110303
Subject(s) - chemical ionization , chemistry , degree of unsaturation , electron ionization , mass spectrometry , selected ion monitoring , atmospheric pressure chemical ionization , chromatography , ionization , analytical chemistry (journal) , gas chromatography , ion , gas chromatography–mass spectrometry , organic chemistry
Mass spectrometric determination of moth pheromone components by means of selected ion monitoring with electron impact and chemical ionization has been evaluated. For the analysis of acetates, chemical ionization with ammonia as reactant gas proved advantageous with respect to sensitivity and selectivity. A quantitative assay for determination of pheromone samples from individual turnip moth females ( Agrotis segetum , Noctuidae), using internal standards, deuterated at the acyl group, was developed. The assay showed correct linearity in the investigated region (20–2000 pg). Signal to noise ratios for 100 pg of acetates using chemical ionization (ammonia as reactant gas) were in the range 10–50 depending on the degree of unsaturation. Conservative limits of detection (background signal + three times the standard deviation) and quantification (background signal + 10 times the standard deviation) were approximately 70 and 240 pg, respectively. A significant decrease in gland titre of ( Z )‐5‐decenyl acetate and ( Z )‐7‐dodecenyl acetate after mating was established.