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The identification of C‐18 neutral steroids in normal stallion urine
Author(s) -
Houghton E.,
Copsey J.,
Dumasia M. C.,
Haywood P. E.,
Moss M. S.,
Teale P.
Publication year - 1984
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200110209
Subject(s) - urine , anabolic steroid , urine collection device , chemistry , physiology , medicine , chromatography , endocrinology , anabolism
As part of a continuing research program associated with the detection of anabolic steroid residues in horse urine, 1–8 normal samples from entire male horses have now been investigated. Isomers of three C‐18 neutral steroids; 4‐estron‐17‐ol‐3‐one (1), estrane‐3,17‐diol (2) and an unsaturated estranediol having a possible structure (3), have been identified in urine samples from two male horses aged 8 and 14 years. Of these three steroids, compound (2) was not detected in the urine of a 2.5 yr old entire male nor in the majority of post‐race urine samples from entire male horses average age 3.8 yrs ( n = 34). Ten of these samples showed tentative indications of this compound. Although the isolation of isomers of estrane‐3,17‐diol from human non‐pregnancy urine has been reported previously, 9 analysis of non‐pregnancy urine samples in the present study did not reveal the presence of these compounds.