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Gas chromatography mass spectrometry of tert ‐butyldimethylsilyl ethers of phthiocerols and mycocerosic alcohols from Mycobacterium tuberculosis
Author(s) -
Mallet A. I.,
Minnikin D. E.,
Dobson G.
Publication year - 1984
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200110207
Subject(s) - derivatization , chemistry , chromatography , mass spectrometry , gas chromatography , gas chromatography–mass spectrometry , extraction (chemistry) , mycobacterium tuberculosis , organic chemistry , tuberculosis , medicine , pathology
Mycobacteria synthesize a variety of unusual long‐chain fatty acid esters and the systematic analysis of these is of great potential in the classification and identification of these bacteria. This paper describes the extraction, reductive fission and derivatization to tert ‐butyldimethylsilyl ethers of β‐diesters, the phthiocerol dimycocerosates. The mass spectra of these ethers are characteristic of the parent alcohols and selected ion monitoring techniques have been applied to mixtures extracted from strains of Mycobacterium tuberculosis .