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Quantitative analysis of prostanoids in biological fluids by combined capillary column gas chromatography negative ion chemical ionization mass spectrometry
Author(s) -
Waddell K. A.,
Barrow S. E.,
Robinson C.,
Orchard M. A.,
Dollery C. T.,
Blair I. A.
Publication year - 1984
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200110205
Subject(s) - chemistry , chromatography , mass spectrometry , gas chromatography , chemical ionization , selected ion monitoring , gas chromatography–mass spectrometry , ion , ionization , organic chemistry
An assay for the quantitative analysis of six biologically important prostanoids based on combined gas chromatography negative ion chemical ionization mass spectrometry has been developed. Prostanoids were extracted from biological fluids by liquid chromatography on Sep‐Pak cartridges and converted to pentafluorobenzyl ester derivatives. Samples were injected on capillary columan by the splitless technique and injections were made in a high boiling hydrocarbon solvent ( n ‐dodecane) in order to minimize chromatographic run times. Quantification was carried out using selected ion monitoring of the appropriate [M‐pentafluorobenzyl] − anion. The assay has been used for profiling cyclooxygenase metabolites of arachidonic acid in guinea pig lung perfusate after induction of anaphylaxis and platelet rich plasma after collagen‐stimulated aggregation.