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Quantitative aspects of fast atom bombardment mass spectrometry
Author(s) -
Beckner Carl F.,
Caprioli Richard M.
Publication year - 1984
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200110203
Subject(s) - analyte , chemistry , mass spectrometry , chromatography , analytical chemistry (journal) , internal standard
Aspects of quantitative analysis using fast atom bombardment mass spectrometry were investigated for several compounds of biological interest. Internal standards of two types were employed; the structural analog dansyl‐asparagine for the quantitation of dansyl‐glutamine and stable isotopically labeled 4‐amino‐(2,2‐ 2 H 2 )butyric acid for the quantitation of 4‐aminobutyric acid. Widely varying concentration ranges, from 0.5 to 250 nmol μl −1 , were measured to establish the dynamic range of the measurements. The results showed that at analyte concentrations below 10–20 nmol μl −1 , the abundance of the analyte's quasi‐molecular ion is proportional to concentration while the abundance of that of the internal standard is relatively constant. At higher concentrations of analyte, the abundance of the [M + H] + of the internal standard decreases while that for the analyte increases, with both of these effects diminishing as the surface of the sample becomes saturated with analyte. Nevertheless, the standard curve produced from plotting the abundance of the [M + H] + of the analyte (relative to that of the internal standard) versus the amount of analyte gives a straight line.