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Direct quantitative determinations by multiple metastable peak monitoring. 1—warfarin in plasma
Author(s) -
Davies Noel W.,
Bignall John C.,
Roberts Michael S.
Publication year - 1983
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200101205
Subject(s) - metastability , chemistry , ion , mass spectrometry , plasma , analytical chemistry (journal) , warfarin , derivatization , instrumentation (computer programming) , chromatography , medicine , physics , organic chemistry , quantum mechanics , computer science , cardiology , operating system , atrial fibrillation
A technique for the direct quantitative determination of plasma warfarin concentrations has been developed using multiple metastable peak monitoring on a double focusing mass spectrometer of normal geometry. The method uses a direct insertion probe and requires no sample purification or derivatization. Deuterated warfarin was added as an internal standard, and the first field free region metastable fragmentations for the loss of an acetyl radical from the molecular ion of both warfarin and internal standard were monitored. These independent daughter ions were focused by a simple combination of linking the magnetic and electric sectors, accelerating voltage switching and selected ion monitoring using standard instrumentation. Results were acquired and processed by normal selected ion monitoring software. Repeated determinations of plasma warfarin concentrations were made with plasma extracts representing approximately 4 μl of plasma, each sample taking two minutes to process by this technique.