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Mixed gas chemical ionization mass spectrometry of peptide derivatives
Author(s) -
Yates John R.,
Anderegg Robert J.
Publication year - 1983
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200101007
Subject(s) - chemistry , chemical ionization , mass spectrometry , mass spectrum , isobutane , reagent , protonation , ion , fast atom bombardment , isoleucine , proton affinity , molecule , ionization , analytical chemistry (journal) , amino acid , chromatography , leucine , organic chemistry , biochemistry , catalysis
In chemical ionization mass spectrometry, the use of a noble reagent gas mixed with a proton transfer reagent gas is known to produce mass spectra which contain both protonated molecules and fragment ions. We have used a mixture of isobutane and argon to improve the mass spectra of peptides which have been derivatized to O ‐TMS‐polyamino alcohols. The molecular weight is deduced from a prominent [M+H] + ion; the sequence of amino acids is deduced from the usual A‐ and Z‐series fragment ions. This technique enhances the sequence ions for lysine and glycine‐containing peptides, and retains the ability to distinguish C‐terminal leucine and isoleucine.