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Sequencing of underivatized peptides by direct liquid inlet liquid chromatography mass spectrometry
Author(s) -
Kenyon Christine N.
Publication year - 1983
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200101002
Subject(s) - chemistry , chromatography , mass spectrometry , acetonitrile , mass spectrum , analytical chemistry (journal) , ion , peptide , resolution (logic) , organic chemistry , biochemistry , artificial intelligence , computer science
Direct liquid inlet liquid chromatography mass spectrometry has been utilized for the detection and sequencing of the peptides lecuine‐enkephalin, methionine‐enkephalin, and alpha‐amanitin. The enkephalins were detected at the nannogram (high picomole) level injected using an RP8 column and a buffer system consisting of ammonium trifluoroacetate and acetonitrile. Positive and negative ion mass spectra were obtained using an RP8 column and a buffer system consisting of triethylammonium acetate and acetonitrile. The sequence of the peptides could be readily determined from the spectra obtained and the positive and negative ion mass spectra provided complementary structural information. A large [M + 1] + or [M‐1] − was obtained in the positive and negative ion modes of operation. A minimal amount of chromatographic resolution was lost upon going from the variable wavelength detector to the mass spectrometer as the detector. This system has the advantage of being a simple and rapid on‐line method, providing sequence information, and being sufficiently sensitive for use in detection of peptides isolated from endogenous sources.