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β‐oxidation of C‐6‐C‐10 fatty acids in rat liver homogenates measured by selected ion monitoring: Effects of cyanide and clofibrate
Author(s) -
Mortensen Per Brøbech,
Rasmussen Karsten
Publication year - 1983
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200100908
Subject(s) - decanoic acid , clofibrate , chemistry , cyanide , hydrolysis , chromatography , substrate (aquarium) , metabolism , fatty acid , biochemistry , organic chemistry , biology , ecology
The β‐oxidation of C‐6‐C‐10‐fatty acids/acyl CoA/acylcarnitines in whole ratliver homogenates was determined by specific and simultaneous measurements of the C‐6‐C‐10‐fatty acids, i.e. hexanoic, octanoic and decanoic acids, in hydrolysed homogenates in relation to time in assays incubated with the above‐mentioned substrates. Measurements were performed by a combined gas chromatographic mass spectrometric technique, i.e. selected ion monitoring. The rate of β‐oxidation of the C‐6‐C‐10‐fatty acids/acyl CoA/acylcarnitines were registered as the consumption rate of the added substrate. The conversion to the C‐2‐shortened β‐oxidation products was illustrated simultaneously. The rate of β‐oxidation of C‐6‐C‐10‐fatty acids was several times higher in homogenates from clofibrate‐treated rats than in control rats, both in the presence and absence of 2.0 mmol l −1 cyanide. Cyanide caused a minor but significant decrease in the β‐oxidation rate in both control and clofibratetreated rats. No differences were found between the β‐oxidation of decanoic acid and decanoyl CoA in homogenates from clofibrate‐treated rats, whereas the degree of β‐oxidation of DL‐decanoylcarnitine was halved compared with decanoic acid and decanoyl CoA.