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Determination of β‐phenylethylamine as its isothiocyanate derivative in biological samples by gas chromatography mass spectrometry
Author(s) -
Suzuki Osamu,
Hattori Hideki
Publication year - 1983
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200100708
Subject(s) - derivative (finance) , chemistry , chromatography , isothiocyanate , derivatization , mass spectrometry , gas chromatography–mass spectrometry , selected ion monitoring , pargyline , gas chromatography , dimethylamine , organic chemistry , monoamine oxidase , financial economics , economics , enzyme
A detailed procedure of a gas chromatographic mass spectrometric assay for β‐phenylethylamine in biological samples, after its reaction with carbon disulphide to form the isothiocyanate derivative, is presented. Our method has advantages over the previous methods with the pentafluoropropionic derivative of β‐phenylethylamine in that the isothiocyanate derivative of β‐phenylethylamine is much more stable than the pentafluoropropionic derivative and that the background in selected ion monitoring is very low. Using the present method, the levels of β‐phenylethylamine in human urine, untreated and pargyline‐treated rat brain were found to be 15.3 μg 24 h −1 , 1.4 and 160 ng g −1 wet weight, respectively.