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A sensitive and specific stable isotope assay for warfarin and its metabolites
Author(s) -
Bush E. D.,
Low L. K.,
Trager W. F.
Publication year - 1983
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200100702
Subject(s) - detection limit , chromatography , chemistry , warfarin , microsome , deuterium , mass spectrometry , isotope , biochemistry , enzyme , medicine , physics , quantum mechanics , cardiology , atrial fibrillation
Abstract A capillary gas chromatographic mass spectrometric method for the quantification of warfarin and its known metabolites from microsomal incubations is described. Deuterium labelled 4′, 6‐, 7‐ and 8‐hydroxy warfarins are used as internal standards and the method has detection limits of 1 ng ml −1 with 20 ng ml −1 being the lower limit for accurate quantification.