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Liquid chromatography mass spectrometry of nucleosides using a commercially available direct liquid introduction probe
Author(s) -
Esmans E. L.,
Luyten Y.,
Alderweireldt F. C.
Publication year - 1983
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200100602
Subject(s) - chemistry , hypoxanthine , chromatography , inosine , uridine , ammonium formate , uracil , mass spectrometry , elution , xanthine , adenosine , organic chemistry , biochemistry , dna , rna , gene , enzyme
Mass spectra were obtained for a series of purine and pyrimidine nucleosides in liquid chromatographic mass spectrometric experiments using a direct liquid introduction probe as interface. Separation of a mixture of adenosine, thymidine, uridine and inosine, and a mixture of hypoxanthine, xanthine, adenine, adenosine, uridine, uracil and thymidine was accomplished using gradient elution with methanol and ammonium formate as a direct liquid introduction liquid chromatographic mass spectrometric compatible solvent system.