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Identification of an acidic metabolite of N ‐nitrosodiethanolamine isolated from rat urine
Author(s) -
Airoldi L.,
Bonfanti M.,
Benfenati E.,
Tavecchia P.,
Fanelli R.
Publication year - 1983
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200100505
Subject(s) - urine , chemistry , metabolite , chromatography , moiety , trimethylsilyl , nitroso , derivative (finance) , medicinal chemistry , organic chemistry , biochemistry , financial economics , economics
N ‐Nitrosodiethanolamine metabolism was studied in order to clarify the mechanism(s) by which this compound elicits its carcinogenic effect. Samples of 24 h urine from rats given a single dose of N ‐nitrosodiethanolamine were collected. Gas chromatographic thermal energy analyses showed the presence of only one compound containing the nitroso moiety besides N ‐nitrosodiethanolamine. This compound was identified as the acidic derivative N ‐(2‐hydroxyethyl)‐ N ‐carboxymethylnitrosamine by comparison with an authentic standard by means of gas chromatographic mass spectrometric analysis of the trimethylsilyl or pentafluorobenzyl esters. The amount of N ‐(2‐hydroxyethyl)‐ N ‐carboxymethylnitrosamine excreted in the 24 h urine was about 6% of the N ‐nitrosodiethanolamine administered.