Identification of an acidic metabolite of  N ‐nitrosodiethanolamine isolated from rat urine | Zendy

Airoldi L. | Zendy; Bonfanti M. | Zendy; Benfenati E. | Zendy; Tavecchia P. | Zendy; Fanelli R. | Zendy

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Identification of an acidic metabolite of N ‐nitrosodiethanolamine isolated from rat urine

Author(s) -

Airoldi L.,

Bonfanti M.,

Benfenati E.,

Tavecchia P.,

Fanelli R.

Publication year - 1983

Publication title -

biomedical mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.475

H-Index - 121

eISSN - 1096-9888

pISSN - 0306-042X

DOI - 10.1002/bms.1200100505

Subject(s) - urine , chemistry , metabolite , chromatography , moiety , trimethylsilyl , nitroso , derivative (finance) , medicinal chemistry , organic chemistry , biochemistry , financial economics , economics

N ‐Nitrosodiethanolamine metabolism was studied in order to clarify the mechanism(s) by which this compound elicits its carcinogenic effect. Samples of 24 h urine from rats given a single dose of N ‐nitrosodiethanolamine were collected. Gas chromatographic thermal energy analyses showed the presence of only one compound containing the nitroso moiety besides N ‐nitrosodiethanolamine. This compound was identified as the acidic derivative N ‐(2‐hydroxyethyl)‐ N ‐carboxymethylnitrosamine by comparison with an authentic standard by means of gas chromatographic mass spectrometric analysis of the trimethylsilyl or pentafluorobenzyl esters. The amount of N ‐(2‐hydroxyethyl)‐ N ‐carboxymethylnitrosamine excreted in the 24 h urine was about 6% of the N ‐nitrosodiethanolamine administered.

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