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The negative ion mass spectra of selected nucleosides
Author(s) -
Smith David L.,
Schram Karl H.,
McCloskey James A.
Publication year - 1983
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200100407
Subject(s) - mass spectrum , ion , mass spectrometry , chemistry , spectral line , chemical ionization , ionization , polyatomic ion , trimethylsilyl , molecule , ion source , analytical chemistry (journal) , organic chemistry , chromatography , physics , astronomy
Negative ion mass spectra of several free nucleosides as well as their trifluoroacetyl, trimethylsilyl, and 2′‐ o ‐nitrobenzyl derivatives are reported. A low ion source pressure is used so that the processes of collisional stabilization and ion/molecule reactions often associated with negative ion chemical ionization do not occur. The utility of such spectra for identification of these and similar nucleosides which may be present as unknowns is discussed. Details of modifications for acquiring negative ion mass spectra using an LKB‐9000S mass spectrometer are given.