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Comparison of radioimmunological determinations with gas chromatography mass spectrometry dosage. A study of PGE 2 and PGF 2α in gastrointestinal fluids
Author(s) -
Bukhave Klaus,
Gréen Krister,
RaskMadsen JøRgen
Publication year - 1983
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200100406
Subject(s) - chromatography , chemistry , selected ion monitoring , mass spectrometry , gas chromatography , radioimmunoassay , sephadex , trimethylsilyl , gas chromatography–mass spectrometry , biochemistry , medicinal chemistry , enzyme
The reliability of radioimmunoassays for determination of PGE 2 and PGF 2α in gastrointestinal fluids was checked by two gas chromatographic mass spectrometric methods. Analyses were performed on samples of gastric juice from dogs and man (healthy volunteers), jejunal fluids from patients with celiac disease, mucous discharge from a villous adenoma of rectum, and bathing solutions from the Ussing chamber containing human jejunal mucosa. The radioimmunoassays included extraction and Sephadex LH‐20 chromatography as a preliminary purification before quantification was carried out. Gas chromatographic mass spectrometric analysis of PGE 2 was performed by monitoring the molecular ion of the trimethylsilyl ether of PGB 2 methyl ester, m / z 420 and m / z 424 for the protium and the deuterium form, respectively, while PGF 2α was quantified as the triacetyl derivative of the methyl ester, using the ion pair (M‐3 × 60) i.e. m / z 314 and m / z 318. Recovery of immunoreactive PGE 2 relative to gas chromatographic mass spectrometric dosage was 88.5% ± 6.5 (mean ± SEM; n = 13), while the amount of PGF 2α in the same sample volume was at the borderline of sensitivity for the gas chromatographic mass spectrometric method used, with a recovery of 114% ± 19 (mean ± SEM; n = 4).