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Targeted class analysis of β‐lactam antibiotics by tandem mass spectrometry
Author(s) -
Barbalas Michael P.,
McLafferty Fred W.,
Occolowitz John L.
Publication year - 1983
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200100404
Subject(s) - chemistry , tandem mass spectrometry , mass spectrum , lactam , electron ionization , mass spectrometry , dissociation (chemistry) , ion , substituent , polyatomic ion , ring (chemistry) , ionization , cleavage (geology) , collision induced dissociation , stereochemistry , computational chemistry , chromatography , organic chemistry , materials science , fracture (geology) , composite material
Another promising application area for tandem mass spectrometry is targeted class analysis. Particularly favorable examples of this are analyses for penicillins and other β‐lactams, important compound classes sought in complex mixtures. Electron ionization of these compounds produces a major fragment ion by lactam ring cleavage; collisionally activated dissociation of this ion yields spectra characteristic of the compound class. Four isomeric [C 7 H 12 NO 2 S] + ions and two homologous ions illustrate these points. Their collisionally activated dissociation spectra, like the corresponding molecular electron ionization spectra, show fragmentations characteristic of the β‐lactam ring size and of substituent type and position, but not of stereochemistry.