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Measurement of bile acid kinetics in human serum using stable isotope labeled chenodeoxycholic acid and capillary gas chromatography electron impact mass spectrometry
Author(s) -
Stellaard Frans,
Schubert Ralf,
Paumgartner Gustav
Publication year - 1983
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200100314
Subject(s) - chenodeoxycholic acid , chemistry , chromatography , mass spectrometry , bile acid , lithocholic acid , gas chromatography , isotope , deoxycholic acid , biochemistry , physics , quantum mechanics
Abstract A non invasive method for measurement of bile acid kinetics in serum using (24‐ 13 C)chenodeoxycholic acid has been developed. After oral administration of 50 mg (24‐ 13 C)chenodeoxycholic acid, the exponential decay of the 13 C atom percent excess was measured in serum using capillary gas chromatography mass spectrometry. This required that isotope ratios were measured with high accuracy and coefficients of variation less than 1% by means of selected ion monitoring and scan averaging. The clinical applicability was tested by repeated determination of pool size, fractional turnover and synthesis rate of chenodeoxycholic acid in one healthy volunteer. This method permitted the determination of pool size, synthesis and conversion of chenodeoxycholic acid into lithocholic acid in man without the use of radioactive tracers and without repeated duodenal intubation.