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Combined capillary column gas chromatography negative ion chemical ionization mass spectrometry of prostanoids
Author(s) -
Waddell K. A.,
Blair I. A.,
Wellby J.
Publication year - 1983
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200100207
Subject(s) - chemistry , mass spectrometry , electron ionization , chromatography , chemical ionization , fragmentation (computing) , ion , mass spectrum , analytical chemistry (journal) , ionization , gas chromatography , ion source , selected ion monitoring , gas chromatography–mass spectrometry , organic chemistry , computer science , operating system
Electron capturing pentafluorobenzyl ester derivatives of prostanoids provide intense negative ion chemical ionization mass spectra. Fragmentation is directed almost entirely away from the prostanoid molecule and this provides intense [M ‐ C 6 F 5 CH 2 ] − ions. These ions are ideal for specific and sensitive quantitative selected ion monitoring analysis. The limits of sensitivity employing this technique are in the range 1–8 pg on column compared with 100–500 pg using the corresponding methyl ester derivative in the electron impact mode. The capillary column characteristics of the pentafluorobenzyl esters are suitable for the development of multiprostaglandin gas chromatographic mass spectrometric assays.