Premium
The combined use of enzymatic hydrolysis and fast atom bombardment mass spectrometry for peptide sequencing
Author(s) -
Self R.,
Parente A.
Publication year - 1983
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200100206
Subject(s) - fast atom bombardment , exopeptidase , mass spectrometry , chemistry , hydrolysis , peptide , oligopeptide , digestion (alchemy) , enzyme , enzymatic hydrolysis , amino acid , chromatography , peptide sequence , biochemistry , gene
Oligopeptides subjected to partial enzyme hydrolysis provide mixtures of sub‐peptides which optimize amino acid sequence determination by fast atom bombardment mass spectrometry. The digestion mixture can be sampled directly; there is no interference from the enzyme. Complete sequences were obtained from spectra recorded on 1–5 nmol samples after digestion at 37°C for times ranging from 0.1–4.0 h. The qualitative results of attack by two exopeptidases on three oligopeptides are discussed.