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Detection and quantification of steroids produced by microbial fermentation of sterols by means of MIKE spectrometry and selected Metastable Ion Monitoring
Author(s) -
Promé D.,
Lacave C.,
Roussel J.,
Promé J. C.
Publication year - 1982
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200091206
Subject(s) - chemistry , chromatography , mass spectrometry , detection limit , ion , metastability , pregnenolone , isobaric process , fermentation , analytical chemistry (journal) , steroid , organic chemistry , biochemistry , physics , hormone , thermodynamics
Several steroids generated by microbial fermentation of cholesterol were identified by the mass analysed ion kinetic energy (MIKE) spectra of their molecular ions generated under electron impact, and quantified by selected metastable ion monitoring. These analyses were performed on a crude solvent extract without any purification or molecular separation procedures. The detection limit was below the nanogram range. Identification and quantification of the major constituents, namely androst‐4‐ene‐3,17‐dione and androsta‐1,4‐diene‐3,17‐dione were fast and easy. Pregnenolone and progesterone which were present at a much lower level were identified with more difficulty by their MIKE spectra alone, because of the existence of isobaric interfering ions. Nevertheless, their quantification by single or dual metastable monitoring was possible.