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The use of capillary column gas chromatography and negative ion chemical ionization mass spectrometry to confirm the administration of synthetic corticosteroids to horses
Author(s) -
Houghton E.,
Teale P.,
Dumasia M. C.,
Wellby J. K.
Publication year - 1982
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200091102
Subject(s) - chemistry , chromatography , mass spectrometry , chemical ionization , mass spectrum , betamethasone , gas chromatography–mass spectrometry , gas chromatography , selected ion monitoring , direct electron ionization liquid chromatography–mass spectrometry interface , ion , analytical chemistry (journal) , ionization , organic chemistry , medicine
The negative ion chemical ionization mass spectra of the MO‐TMS derivatives of the corticosteroids prednisolone, betamethasone and dexamethasone have been obtained using capillary column gas chromatography mass spectrometry. The spectra showed abundant diagnostic ions at m / z >300 allowing for clear discrimination between the three steroid derivatives. A capillary column gas chromatographic mass spectrometric method using negative ion chemical ionization mass spectrometry has been developed to confirm the presence of the parent steroids in horse urine following the administration of proprietary preparations of prednisolone and betamethasone.