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The accurate determination of serum glucose by isotope dilution mass spectrometry—two methods
Author(s) -
White V E.,
Welch M. J.,
Sun T.,
Sniegoski L. T.,
Schafter R.,
Hertz H. S.,
Cohen A.
Publication year - 1982
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200090907
Subject(s) - isotope dilution , chromatography , chemistry , mass spectrometry , dilution , gas chromatography , isotope , analytical chemistry (journal) , gas chromatography–mass spectrometry , physics , quantum mechanics , thermodynamics
Two isotope dilution mass spectrometric methods have been developed for the determination of D‐glucose in human serum. Each uses a uniformly labeled ( 13 C)glucose as the internal standard. The first method involves conversion of glucose into 1,2: 5,6‐di‐ O ‐isopropylidene‐α‐ D ‐glucofuranose and an extensive clean‐up, followed by quantitation using packed column gas chromatography mass spectrometry. In the second method, glucose is converted into α‐ D ‐glucofuranose cyclic 1,2: 3,5‐bis(butylboronate)‐6‐acetate. The wet chemistry work‐up is simpler, but analysis by capillary gas chromatography mass spectrometry is required. Both methods exhibit excellent precision (coefficients of variation <0.3%) and provided mean values that agree within 1% for all serum pools tested.