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Field desorption mass spectrometry of cobalamine derivatives mixture analysis thermolytic reactions and comparison with fast atom bombardment
Author(s) -
Schiebel H. M.,
Schulten H.R.
Publication year - 1982
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200090809
Subject(s) - chemistry , corrin , fast atom bombardment , mass spectrometry , desorption , fragmentation (computing) , thermal decomposition , substituent , analytical chemistry (journal) , mass spectrum , field desorption , ion , ring (chemistry) , organic chemistry , chromatography , adsorption , computer science , ionization , operating system
For cobyrinic acid heptamethylester derivatives, field desorption mass spectrometry can be used as a fast and sensitive analytical method for synthesis control in corrin chemistry. Since molecular ions are formed with high relative abundance, their identification in mixtures using accurate mass measurements and direct isotopic determination is feasible. Detailed structural information can be obtained by thermally‐induced fragmentation of the organometallic compounds on the emitter surface. Consecutive loss of the axial ligands and the substituent in position 10 of the corrin ring is observed with increasing sample temperature. Furthermore, at higher emitter heating currents, the product of the elimination of the acetic acid side‐chain from C‐7 is found analogously as in preparative thermolysis. The field desorption study presented here allows not only the detection of thermally generated end‐products but also of reactive intermediates and thus gives a specific insight into the mechanisms of thermolysis. A preliminary comparison of the field desorption and fast atom bombardment mass spectra of some corrins is presented.