Premium
Quantitative analysis of 5‐fluorouracil and 5,6‐dihydrofluorouracil in plasma by gas chromatography mass spectrometry
Author(s) -
Aubert C.,
Sommadossi J. P.,
Coassolo Ph.,
Cano J. P.,
Rigault J. P.
Publication year - 1982
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200090806
Subject(s) - fluorouracil , mass spectrometry , chemistry , chromatography , detection limit , diethyl ether , gas chromatography , extraction (chemistry) , gas chromatography–mass spectrometry , quantitative analysis (chemistry) , electron ionization , ionization , ion , medicine , organic chemistry , chemotherapy
Abstract 5‐Fluorouracil and 5,6‐dihydro‐5‐fluorouracil were analysed in the plasma of patients by combined gas chromatography mass spectrometry. 5‐Bromouracil was the internal standard. After extraction from plasma with an isopropanol–diethyl ether mixture (20/80) the components were pentylated and the derivatives produced extracted into diethyl ether. Electron impact mass spectrometry was used for the simultaneous quantitative determinations of 5‐fluorouracil and 5,6‐dihydro‐5‐fluorouracil (detection limit 10 ng mI −1 5‐fluorouracil, 80 ng mI −1 5,6‐dihydro‐5‐fluorouracil). Chemical ionization was utilized to measure 5,6‐dihydro‐5‐fluorouracil concentrations <80 ng mI −1 (sensitivity 10 ng mI −1 ). The biological applicability of these two techniques was demonstrated by analysing plasma samples from patients after administration of 5‐fluorouracil or 5′‐deoxyfluorouridine by intravenous injections and infusions.