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Effects of 20,25‐diazacholesterol on cholesterol synthesis in cultured chick muscle cells: A radiogas chromatographic and mass spectrometric study of the post‐squalene sector
Author(s) -
Emmons G. T.,
Rosenblum E. R.,
Peace J. N.,
Malloy J. M.,
Doerfler D. L.,
McManus I. R.,
Campbell I. M.
Publication year - 1982
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200090703
Subject(s) - lanosterol , squalene , demethylation , sterol , double bond , chemistry , biosynthesis , biochemistry , enzyme , cholesterol , chromatography , isomerase , stereochemistry , organic chemistry , gene expression , dna methylation , gene
Abstract Radiogas chromatography, used in conjunction with mass spectrometry, has been used to analyze the sterol content of cultured chick muscle cells. Seven sterols, plus lanosterol, were detected. These sterols conformed to a linear biosynthetic pathway linking lanosterol and cholesterol. The reaction sequence is: C‐14 demethylation, C‐4 demethylation, Δ 8 → Δ 5 double bond rearrangement, Δ 24 double bond reduction. When chick cells were treated with increasing concentrations of 20,25‐diazacholesterol, components of this pathway and aberrant products accumulated. These accumulations suggest that diazacholesterol affects reductases, double bond isomerases and the C‐14 demethylation enzymes of sterol biosynthesis.