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Secondary ion mass spectra of the neuropeptides leucine‐enkephalin and methionine‐enkephalin
Author(s) -
Westmore J. B.,
Ens W.,
Standing K. G.
Publication year - 1982
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200090309
Subject(s) - ion , chemistry , mass spectrum , leucine , methionine , peptide , amino acid , mass spectrometry , spectral line , molecule , analytical chemistry (journal) , biochemistry , chromatography , physics , organic chemistry , astronomy
Positive and negative secondary ion mass spectra obtained by bombardment of thin films of leucine‐enkephalin and methionine‐enkephalin with a pulsed beam of Cs + ions of energy 5–15 keV were recorded using a time‐of‐flight mass spectrometer. Many of the secondary ions are metastable. The spectra contain a wealth of information, including the following important features: (i) ions characteristic of the intact molecule, from which the molecular weight may be deduced; (ii) ions diagnostic of the amino acid composition of the peptide; (iii) ions diagnostic of the sequence of amino acids in the peptide.