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Quantitative determination of nitroglycerin in human plasma by capillary gas chromatography negative ion chemical ionization mass spectrometry
Author(s) -
Ottoila Pekka,
Taskinen Jyrki,
Sothmann Aslak
Publication year - 1982
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200090306
Subject(s) - chemistry , chromatography , mass spectrometry , calibration curve , chemical ionization , detection limit , nitroglycerin (drug) , analytical chemistry (journal) , gas chromatography , pentane , ion , quantitative analysis (chemistry) , ionization , gas chromatography–mass spectrometry , capillary action , materials science , medicine , organic chemistry , anesthesia , composite material
A quantitative method for determination of nitroglycerin in human plasma was developed. Nitroglycerin and the internal standard (butane‐1,2,4‐triyl trinitrate) were extracted from plasma with pentane. The extracts were analysed by gas chromatography mass spectrometry using fused silica capillary columns and electron capture negative ion chemical ionization. The quantitation limit of the method was about 50 pg ml −1 . Linear calibration curves were obtained in the range of 50–1600 pg ml −1 . Precision at the level of 100 pg ml −1 was 4%.