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Quantitative profiling analysis of organic acids in complex mixtures
Author(s) -
Sweeley Charles C.,
Vrbanac James,
Pinkston David,
Issachar David
Publication year - 1981
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200080915
Subject(s) - chromatography , chemistry , profiling (computer programming) , mass spectrometry , capillary action , gas chromatography , materials science , computer science , composite material , operating system
The simultaneous detection and quantitative determination of a very large number of metabolites in physiological fluids or tissues, and the subsequent comparison of these data with reference values, is a process which has commonly been called “metabolic profiling” analysis. Profiles generated by gas‐liquid chromatography on packed or capillary columns are readily analyzed by mass spectrometry, utilizing reverse library search procedures to identify individual components. Emphasis will be given here to procedures for the pre‐purification of organic acids from plasma, to the use of quartz capillaries for metabolic profiling analysis, and to the sensitivity of this method for trace components.