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Sequence analysis of polypeptides by collision activated dissociation on a triple quadrupole mass spectrometer
Author(s) -
Hunt Donald F.,
Buko Alexander M.,
Ballard John M.,
Shabanowitz Jeffrey,
Giordani Anne B.
Publication year - 1981
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200080909
Subject(s) - triple quadrupole mass spectrometer , mass spectrometry , dissociation (chemistry) , quadrupole , hybrid mass spectrometer , collision , quadrupole mass analyzer , spectrometer , sequence (biology) , chemistry , physics , atomic physics , selected reaction monitoring , computer science , tandem mass spectrometry , chromatography , optics , biochemistry , computer security
A new approach to the direct sequencing of oligopeptides in complex mixtures is described. Mixtures of [ 2 H 0 ]/[ 2 H 3 ]‐ N ‐acetylated and N, O ‐permethylated peptides are analyzed by collision activated dissociation on a triple quadrupole mass spectrometer using isobutane chemical ionization. Analysis of the collision activated dissociation spectra enables peptide sequences to be deduced. Use of electron capture negative chemical ionization for the sequence analysis of neuropeptides at the picomole level is also described.