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A two‐dimensional analysis of variance for the simultaneous quantitative determination of estradiol and its 4‐ 14 C isotopically labelled analogue with selected ion monitoring
Author(s) -
Louton Tom,
Schill W.,
Schramm W.
Publication year - 1981
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200080607
Subject(s) - derivatization , chromatography , mass spectrometry , chemistry , analytical chemistry (journal) , gas chromatography–mass spectrometry , titration , gas chromatography , quantitative analysis (chemistry) , analysis of variance , selected ion monitoring , mathematics , statistics , inorganic chemistry
Estradiol‐17β and [4‐ 14 C]estradiol‐17β in total mass amounts of 0, 10 and 20 pg per analysis in the presence of 1000 pg [ 2 H 8 ]estradiol as an internal standard were simultaneously quantitatively determined by gas chromatography mass spectrometry using the selected ion monitoring technique. The experiment (a nested three way analysis of variance) was designed to investigate the two‐dimensional error structure which facilitates the study and comparison of the variances: (1) the variance originating in the device, including gas chromatograph, mass spectrometer and computer system with its interfaces; (2) the variance originating from titration, pipetting and derivatization of the samples. The study revealed that in this concentration range the variance due to sample preparation is smaller than that due to the device. The deviations due to the device were shown to have a two‐dimensional normal distribution.