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Simultaneous measurement of plasma 4‐hydroxy‐3‐methoxyphenylethlene glycol and 3,4‐dihydroxyphenylethylene glycol by gas chromatography mass spectrometry
Author(s) -
Jimerson D. C.,
Markey S. P.,
Oliver J. A.,
Kopin I. J.
Publication year - 1981
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200080606
Subject(s) - chemistry , chromatography , mass spectrometry , dichloromethane , gas chromatography , gas chromatography–mass spectrometry , solvent , derivatization , ethylene glycol , organic chemistry
A sensitive gas chromatographic mass spectrometric assay has been developed to measure plasma 4‐hydroxy‐3‐methoxyphenylethylene glycol and 3,4‐dihydroxyphenylethylene glycol. Following addition of deuterated internal standards, the metabolites are propinylated to facilitate extraction with dichloromethane. After evaporation of the solvent, the residue is derivatized with t ‐butyldimethysilmidazole. The reaction product is washed with water, evaporated to dryness, and reconstituted with hexane for gas chromatography mass spectrometry. The single major fragment ion from each of the derivatized metabolites is monitored, yielding a sensitivity of approximately 3‐5 pmol ml −1 . Improved precision for the 3,4‐dihydroxyphenylethylene glycol assay may be facilitated by synthesis of a more stable internal standard.