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Structural analysis of mannans from Candida albicans serotypes A and B and from Torulopsis glabrata by methylation gas chromatography mass spectrometry and exo‐α ‐mannanse
Author(s) -
Reiss E.,
Patterson D. G.,
Yert L. W.,
Holler J. S.,
Ibrahim B. K.
Publication year - 1981
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200080605
Subject(s) - methylation , mass spectrometry , serotype , chromatography , gas chromatography , chemistry , gas chromatography–mass spectrometry , microbiology and biotechnology , biology , biochemistry , gene
Mannans of Candida albicans serotypes A and B and of Torulopsis glabrata were subjected to methylation analysis and the resulting monosaccharides were converted to the peracetylaldonitrile derivatives and subsequently analyzed by gas chromatography mass spectrometry. Serotype A differed from B in having more 1→2 linked mannosyl residues (46.2 vs 37.1 mol%) indicating longer oligomannosyl sidechains; mannosyl branch points linked through C‐1, C‐3 and C‐6 were detected for the first time in C. albicans mannans and were more abundant in serotype B (9.2 vs 5.3 mol%). T. glabrata mannan differed from that of C. albicans in having virtually no (1.6 mol%) unsubsituted sugars in the linear backbone and less 1→2 linked mannosyl residues (33.1 mol%) interpreted as shorter oligomannosyl sidechains. Model building of a repeating unit for the outer chain region of these mannase was prevented by the large amount of nonreducing terminal mannosyl residues, 23.0 mol% for C. albicans A, that probably arise from the inner core region. These mannans were exposed to exo‐α ‐mannanase and the percentage of digestion, measured as reducing sugar released, was 33.5% for C. albicans B ; 27.4% for T. glabrata , and C. albicans A was refractroy (5.4% digested).