Premium
Detection of urinary primary amines through negative chemical ionization mass spectrometry of fluorescamine derivatives
Author(s) -
Bergner E. A.,
Dougherty Ralph C.
Publication year - 1981
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200080504
Subject(s) - fluorescamine , chemistry , derivatization , chromatography , chemical ionization , mass spectrometry , tyramine , normetanephrine , organic chemistry , ionization , urine , biochemistry , ion , physics , quantum mechanics , fluorescence
We report the successful detection of urinary biogenic amines by a procedure which involves isolation of the amines on CG‐50 cation exchange resin, derivatization of the amines with fluorescamine while on the resin, followed by direct probe negative chemical ionization mass spectrometry screening of the resin extract. This isolation procedure allowed the detection of tyramine, octopamine, normetanephrine, 3‐methoxytyramine and a variety of aliphatic amines. Norepinephrine, dopamine and serotonin were chemically reactive under these conditions and were not detectable at physiological concentrations.