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Determination of glutamic acid and ω‐amino‐butyric acid in Ringer's solution without desalination at the femtomole level by gas chromatography chemical ionization mass spectrometry
Author(s) -
Murayama Kimie,
Shindo Noriko,
Mineki Reiko,
Ohta Keiko
Publication year - 1981
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200080407
Subject(s) - chemistry , chromatography , trifluoroacetic anhydride , acylation , sodium , mass spectrometry , butyric acid , sodium acetate , gas chromatography , acetic anhydride , carbonic acid , gas chromatography–mass spectrometry , chemical ionization , nuclear chemistry , organic chemistry , ionization , catalysis , ion
For the quantification of glutamic acid in Ringer's solution, pentafluoropropionic methyl ester was the most sensitive derivative. The detectable concentration was 0.01 m̈M glutamic acid in Ringer's solution; the amount of the preparation was 1 pmol and the injection into a gas chromatograph mass spectrometer was 10 fmol. For the quantification of m̈‐aminobutyric acid in Ringer's solution, the trifluoroacetal‐hexafluoropropionyl ester was detectable at a concentration of 0.01 m̈M. Ringer's salts facilitated acylation in the order heptafluorobutyric anhydride > pentafluoropropionic anhydride > trifluoroacetic anhydride. The effect depended on esterification of carboxy groups in the order methyl ester > hexafluoropropionyl ester > butyl ester. Sodium carbonate, sodium acetate and sodium citrate also facilitated acylation with pentafluoropropionic anhydride, while sodium phosphate inhibited the acylation and sodium sulfate inhibited it slightly. The pentafluoropropionic methyl ester of glutamic acid was stable for up to 10 days, when it was dissolved in acetone and stored at–8°C.