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Mass spectrometry of permethylated folic acid analogs
Author(s) -
Smith Ronald G.,
Pegues Joyce C.,
Farquhar David,
Loo Ti Li,
Wang YeuMing
Publication year - 1981
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200080403
Subject(s) - chemistry , folic acid , mass spectrometry , methotrexate , mass spectrum , urine , ion , amino acid , chromatography , stereochemistry , biochemistry , organic chemistry , medicine , immunology , biology
Folic acid and several analogs have been characterized as their permethylated derivatives. These derivatives are readily evaporated from the solid probe and provide spectra with abundant molecular ions and characteristic fragment ions. This method has been used to confirm the identities of the two methotrexate metabolites, 7‐hydroxymethotrexate and 4‐amino‐4‐deoxy‐N 10 ‐methylpteroic acid, which have been isolated from human urine. The permethylation products of the folate cofactors, dihydrofolic acid, tetrahydrofolic acid and leucovorin, are also characterized.